Composite

Part:BBa_K1040005:Design

Designed by: Taku Tsuzuki   Group: iGEM13_Osaka   (2013-09-17)


RBS(BBa_B0034)+ilvE(BBa_K1040000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 162
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

  • BBa_B0034(RBS)
  • [http://www.ncbi.nlm.nih.gov/nuccore/X04890.1 E.coli ilvGMEDA operon]

References

  • Lawther RP, Wek RC, Lopes JM, Pereira R, Taillon BE, Hatfield GW.,The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12.,Nucleic Acids Res. 1987 Mar 11;15(5):2137-55.
  • K Hosoda,Cooperative Adaptation to Establishment of a Synthetic Bacterial Mutualism.(2011)